Instruction for Use of Supplemental Table 1
Click Download Data below and you will access the (tab delimited) data on the web. Save as text file (xxxx.txt). Then quit the internet software.
Open excel software and open the above data file using the menu option (Go to File/open).
The text import wizard will automatically open. Leave every option as it is (as the default) and keep pressing the next button till you get to the last step and press finish. Then the excel file will open. Save as an excel worksheet format on your desktop and use this file to browse the data.
For browsing the data, it is convenient to use the filter function. First select the entire data by clicking on upper left space (above number one) on excel file and go to data/filter/autofilter in the excel menu bar and click the arrow heads on the first row of the gene name column and select custom filter and a custom autofilter window will appear. Select words (e.g., contains, equals, etc.) for rows and type in the key words (e.g., ion channel, receptor, or specific molecules, etc.) and hit OK, then you will get a selected number of genes and data according to your keywords. To begin a new search, click on arrow head again and select “show all” the entire data will appear again.
Supplemental Table 1: The 2909 genes shown in this table decreased in the SON in hypoosmolar as compared to the control conditions. Differences in gene expression were measured by comparative analysis of laser captured control rat SONs (n=3/group) against a rat hypothalamus reference (n=10), with triplicate or quadruplicate array hybridization for each. A significant change in differential expression was accepted only for genes that passed our quality criteria (see Mutsuga et al, 2005) including significant p values (p<0.05) between control and hypoosmolar in the ANOVA analysis. The expression ratios shown here are the SON/Hypothalamus ratios in the control group. Annotations for clone IDs should be reconfirmed by BLAST searches.